Novel markers for high-throughput protoplast-based analyses of phytohormone signaling.
Identifieur interne : 000097 ( Main/Exploration ); précédent : 000096; suivant : 000098Novel markers for high-throughput protoplast-based analyses of phytohormone signaling.
Auteurs : Silke Lehmann [Royaume-Uni] ; Ana Dominguez-Ferreras [Royaume-Uni] ; Wei-Jie Huang [Royaume-Uni] ; Katherine Denby [Royaume-Uni] ; Vardis Ntoukakis [Royaume-Uni] ; Patrick Sch Fer [Royaume-Uni]Source :
- PloS one [ 1932-6203 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- cytologie : Arabidopsis, Protoplastes.
- métabolisme : Facteur de croissance végétal, Marqueurs biologiques.
- Transduction du signal.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Biomarkers, Plant Growth Regulators.
- cytology : Arabidopsis, Protoplasts.
- Signal Transduction.
Abstract
Phytohormones mediate most diverse processes in plants, ranging from organ development to immune responses. Receptor protein complexes perceive changes in intracellular phytohormone levels and trigger a signaling cascade to effectuate downstream responses. The in planta analysis of elements involved in phytohormone signaling can be achieved through transient expression in mesophyll protoplasts, which are a fast and versatile alternative to generating plant lines that stably express a transgene. While promoter-reporter constructs have been used successfully to identify internal or external factors that change phytohormone signaling, the range of available marker constructs does not meet the potential of the protoplast technique for large scale approaches. The aim of our study was to provide novel markers for phytohormone signaling in the Arabidopsis mesophyll protoplast system. We validated 18 promoter::luciferase constructs towards their phytohormone responsiveness and specificity and suggest an experimental setup for high-throughput analyses. We recommend novel markers for the analysis of auxin, abscisic acid, cytokinin, salicylic acid and jasmonic acid responses that will facilitate future screens for biological elements and environmental stimuli affecting phytohormone signaling.
DOI: 10.1371/journal.pone.0234154
PubMed: 32497144
PubMed Central: PMC7272087
Affiliations:
Links toward previous steps (curation, corpus...)
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University of Warwick, Coventry, England</wicri:regionArea><wicri:noRegion>England</wicri:noRegion></affiliation></author><author><name sortKey="Ntoukakis, Vardis" sort="Ntoukakis, Vardis" uniqKey="Ntoukakis V" first="Vardis" last="Ntoukakis">Vardis Ntoukakis</name><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, The University of Warwick, Coventry, England, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Life Sciences, The University of Warwick, Coventry, England</wicri:regionArea><wicri:noRegion>England</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Warwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, England, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Warwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, England</wicri:regionArea><wicri:noRegion>England</wicri:noRegion></affiliation></author><author><name sortKey="Sch Fer, Patrick" sort="Sch Fer, Patrick" uniqKey="Sch Fer P" first="Patrick" last="Sch Fer">Patrick Sch Fer</name><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, The University of Warwick, Coventry, England, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Life Sciences, The University of Warwick, Coventry, England</wicri:regionArea><wicri:noRegion>England</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Warwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, England, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Warwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, England</wicri:regionArea><wicri:noRegion>England</wicri:noRegion></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (cytology)</term><term>Biomarkers (metabolism)</term><term>Plant Growth Regulators (metabolism)</term><term>Protoplasts (cytology)</term><term>Signal Transduction (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arabidopsis (cytologie)</term><term>Facteur de croissance végétal (métabolisme)</term><term>Marqueurs biologiques (métabolisme)</term><term>Protoplastes (cytologie)</term><term>Transduction du signal (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Biomarkers</term><term>Plant Growth Regulators</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Arabidopsis</term><term>Protoplastes</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Arabidopsis</term><term>Protoplasts</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteur de croissance végétal</term><term>Marqueurs biologiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Signal Transduction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Transduction du signal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytohormones mediate most diverse processes in plants, ranging from organ development to immune responses. Receptor protein complexes perceive changes in intracellular phytohormone levels and trigger a signaling cascade to effectuate downstream responses. The in planta analysis of elements involved in phytohormone signaling can be achieved through transient expression in mesophyll protoplasts, which are a fast and versatile alternative to generating plant lines that stably express a transgene. While promoter-reporter constructs have been used successfully to identify internal or external factors that change phytohormone signaling, the range of available marker constructs does not meet the potential of the protoplast technique for large scale approaches. The aim of our study was to provide novel markers for phytohormone signaling in the Arabidopsis mesophyll protoplast system. We validated 18 promoter::luciferase constructs towards their phytohormone responsiveness and specificity and suggest an experimental setup for high-throughput analyses. We recommend novel markers for the analysis of auxin, abscisic acid, cytokinin, salicylic acid and jasmonic acid responses that will facilitate future screens for biological elements and environmental stimuli affecting phytohormone signaling.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32497144</PMID><DateCompleted><Year>2020</Year><Month>08</Month><Day>26</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>26</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>6</Issue><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Novel markers for high-throughput protoplast-based analyses of phytohormone signaling.</ArticleTitle><Pagination><MedlinePgn>e0234154</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0234154</ELocationID><Abstract><AbstractText>Phytohormones mediate most diverse processes in plants, ranging from organ development to immune responses. Receptor protein complexes perceive changes in intracellular phytohormone levels and trigger a signaling cascade to effectuate downstream responses. The in planta analysis of elements involved in phytohormone signaling can be achieved through transient expression in mesophyll protoplasts, which are a fast and versatile alternative to generating plant lines that stably express a transgene. While promoter-reporter constructs have been used successfully to identify internal or external factors that change phytohormone signaling, the range of available marker constructs does not meet the potential of the protoplast technique for large scale approaches. The aim of our study was to provide novel markers for phytohormone signaling in the Arabidopsis mesophyll protoplast system. We validated 18 promoter::luciferase constructs towards their phytohormone responsiveness and specificity and suggest an experimental setup for high-throughput analyses. We recommend novel markers for the analysis of auxin, abscisic acid, cytokinin, salicylic acid and jasmonic acid responses that will facilitate future screens for biological elements and environmental stimuli affecting phytohormone signaling.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lehmann</LastName><ForeName>Silke</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0001-6838-7422</Identifier><AffiliationInfo><Affiliation>School of Life Sciences, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Warwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dominguez-Ferreras</LastName><ForeName>Ana</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>School of Life Sciences, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Warwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Wei-Jie</ForeName><Initials>WJ</Initials><Identifier Source="ORCID">0000-0001-5208-6713</Identifier><AffiliationInfo><Affiliation>School of Life Sciences, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Warwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Denby</LastName><ForeName>Katherine</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>School of Life Sciences, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Warwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ntoukakis</LastName><ForeName>Vardis</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>School of Life Sciences, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Warwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schäfer</LastName><ForeName>Patrick</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>School of Life Sciences, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Warwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, England, United Kingdom.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>BB/M017982/1</GrantID><Acronym>BB_</Acronym><Agency>Biotechnology and Biological Sciences Research Council</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010937">Plant Growth 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